Aerobic Exercise Relieves Cardiac Inflammation by Modulating AP1/JNK Genes in Diet-Induced Obesity Rat Model

Abstract

The obesity has increased the risk of cardiovascular diseases. The purpose of this research is to investigate the effect of aerobic exercise in cardiac inflammation in the experimental model of obesity induced by high-fat and fructose diet. Twenty-four Wistar rats were randomly divided into three groups including, Control, OB: high fat/fructose diet (60% fat and 25% fructose) group and OB+EX: obesity+aerobics exercise group. At the end of the study, concentration level of CRP, amyloid A, fibrinogen, pro/anti-inflammatory cytokines, and also the expression level of AP1 and JNK genes were assessed in all groups. High-fat/fructose diet increased the serum level of inflammatory biomarkers compared to the control group. The concentration level of TNF-a and IL-6 significantly increases in compared to the control rats. Conversely, the level of IL-10 significantly decreases compared to the control group. These results were in line with increases the genes expression of AP1 and JNK compared to the control rats. although, aerobic exercise showed the significant efficacy with prevent inflammatory process in cardiac system which demonstrated by down regulation of inflammatory genes and increases of anti-inflammatory cytokines. According to the obtained data, it seems that aerobic exercise could modulate the inflammatory cascade in cardiac system with suppress the expression of AP1 and JNK genes and improve inflammatory biomarkers in cardiac tissue.